NAD+ injection dosage chart
100, 500 and 1,000 mg vials at four water volumes, every reported dose in units, and what per day, per week and each frequency actually costs in vials.
25 printable pages: dosing cards for the twelve most-used compounds at three water volumes, titration planners, a vial log, and five pages on what to do when it has already gone wrong.
NAD+ is a coenzyme rather than a peptide, but it is sold by the same vendors as a lyophilised powder and the arithmetic is identical. Vials are large, 100, 500 or 1,000 mg, and doses are in tens of milligrams, so the concentrations are far higher than anything else on this site. It is not an approved injectable medicine. Flushing, nausea and pain at the injection site are widely reported, and the pain tracks concentration, which is the one reason here to use more water than the draw alone would need.
100 mg vial
| BAC water | Concentration | 25 mg | 50 mg | 75 mg | 100 mg | 150 mg | 200 mg |
|---|---|---|---|---|---|---|---|
| 1 mL | 100 mg/mL | 25 | 50 | 75 | 100 | - | - |
| 2 mL | 50 mg/mL | 50 | 100 | 150* | 200* | - | - |
| 3 mL | 33.33 mg/mL | 75 | 150* | 225* | 300* | - | - |
| 5 mL | 20 mg/mL | 125* | 250* | 375* | 500* | - | - |
500 mg vial
| BAC water | Concentration | 25 mg | 50 mg | 75 mg | 100 mg | 150 mg | 200 mg |
|---|---|---|---|---|---|---|---|
| 1 mL | 500 mg/mL | 5 | 10 | 15 | 20 | 30 | 40 |
| 2 mL | 250 mg/mL | 10 | 20 | 30 | 40 | 60 | 80 |
| 3 mL | 166.67 mg/mL | 15 | 30 | 45 | 60 | 90 | 120* |
| 5 mL | 100 mg/mL | 25 | 50 | 75 | 100 | 150* | 200* |
1000 mg vial
| BAC water | Concentration | 25 mg | 50 mg | 75 mg | 100 mg | 150 mg | 200 mg |
|---|---|---|---|---|---|---|---|
| 1 mL | 1000 mg/mL | 2.5 | 5 | 7.5 | 10 | 15 | 20 |
| 2 mL | 500 mg/mL | 5 | 10 | 15 | 20 | 30 | 40 |
| 3 mL | 333.33 mg/mL | 7.5 | 15 | 22.5 | 30 | 45 | 60 |
| 5 mL | 200 mg/mL | 12.5 | 25 | 37.5 | 50 | 75 | 100 |
Units on a standard U-100 insulin syringe. * means more than 100 units, so more than one 1 mL syringe. A dash means the dose is larger than the vial.
The 500 mg vial, in detail
A 500 mg vial with 2 mL of bacteriostatic water is 250 mg/mL, so 50 mg is 0.2 mL, or 20 units, and the vial holds 10 doses of that size. It is the most common vial and the most common setup, which is why it is worth its own table.
| BAC water | Concentration | 25 mg | 50 mg | 75 mg | 100 mg | 150 mg | 200 mg |
|---|---|---|---|---|---|---|---|
| 1 mL | 500 mg/mL | 5 | 10 | 15 | 20 | 30 | 40 |
| 2 mL | 250 mg/mL | 10 | 20 | 30 | 40 | 60 | 80 |
| 3 mL | 167 mg/mL | 15 | 30 | 45 | 60 | 90 | 120* |
| 4 mL | 125 mg/mL | 20 | 40 | 60 | 80 | 120* | 160* |
| 5 mL | 100 mg/mL | 25 | 50 | 75 | 100 | 150* | 200* |
500 mg in 2 mL, dose by dose
| Dose | Draw | U-100 units | Doses per vial | Lasts, at once daily |
|---|---|---|---|---|
| 25 mg | 0.1 mL | 10 units | 20 | 20 days |
| 50 mg | 0.2 mL | 20 units | 10 | 10 days |
| 75 mg | 0.3 mL | 30 units | 6 | 6 days |
| 100 mg | 0.4 mL | 40 units | 5 | 5 days |
| 150 mg | 0.6 mL | 60 units | 3 | 3 days |
| 200 mg | 0.8 mL | 80 units | 2 | 2 days |
NAD+ dosage per day
Commonly reported daily amounts sit at 50 to 100 mg, which from a 500 mg vial in 2 mL is 20 to 40 units. Reports generally describe starting at the low end, often 25 mg, and working up as tolerance to the flushing and the sting allows. The wider range the presets cover is 25 to 200 mg. There is no licensed daily dose; this is a description of what gets reported, not a recommendation.
| Daily dose | U-100 units | Draw | Per week | 500 mg vial lasts | 500 mg vials per 30 days |
|---|---|---|---|---|---|
| 25 mg daily | 10 units | 0.1 mL | 175 mg | 20 days | 1.5 |
| 50 mg daily | 20 units | 0.2 mL | 350 mg | 10 days | 3 |
| 75 mg daily | 30 units | 0.3 mL | 525 mg | 6 days | 4.5 |
| 100 mg daily | 40 units | 0.4 mL | 700 mg | 5 days | 6 |
| 150 mg daily | 60 units | 0.6 mL | 1050 mg | 3 days | 9 |
| 200 mg daily | 80 units | 0.8 mL | 1400 mg | 2 days | 12 |
NAD+ dosage per week
At the commonly reported 50 mg a day the weekly total is 350 mg, and at 100 mg a day it is 700 mg, so a 500 mg vial is roughly a week and a half at the low end and five days at the high end. Run three times a week instead and the same per-dose amounts come to 150 and 300 mg a week. The table gives the weekly total, the vial burn and the units for each pattern, all from a 500 mg vial in 2 mL (250 mg/mL).
| Dose | Frequency | U-100 units | Per week | 500 mg vials per week |
|---|---|---|---|---|
| 25 mg | daily | 10 units | 175 mg | 0.35 |
| 50 mg | daily | 20 units | 350 mg | 0.7 |
| 100 mg | daily | 40 units | 700 mg | 1.4 |
| 50 mg | three times a week | 20 units | 150 mg | 0.3 |
| 100 mg | three times a week | 40 units | 300 mg | 0.6 |
| 150 mg | twice weekly | 60 units | 300 mg | 0.6 |
| 200 mg | twice weekly | 80 units | 400 mg | 0.8 |
NAD+ injection frequency
The two patterns that get reported are once daily and three times a week, usually in blocks of one to two weeks rather than continuously, with larger amounts spread over more injections rather than taken in one. Frequency is also where the site pain shows up: the same weekly total split into more, smaller, more dilute injections is the change people describe making when the sting is the problem. Nothing here is a schedule anyone has licensed, and how often is a clinical question, not an arithmetic one.
| Frequency | Dose | U-100 units | Doses per 500 mg vial | Vial lasts |
|---|---|---|---|---|
| Once daily | 50 mg | 20 units | 10 | 10 days |
| Once daily | 100 mg | 40 units | 5 | 5 days |
| Every other day | 100 mg | 40 units | 5 | 10 days |
| Three times a week | 100 mg | 40 units | 5 | 12 days |
| Twice weekly | 150 mg | 60 units | 3 | 10 days |
| Once weekly | 200 mg | 80 units | 2 | 14 days |
Why NAD+ numbers look wrong at first
Every other compound on this site is dosed in milligrams or micrograms out of a vial holding a few milligrams. NAD+ is dosed in tens of milligrams out of a vial holding hundreds, so the concentrations are enormous by comparison: 500 mg in 2 mL is 250 mg/mL, where a 10 mg peptide vial in 2 mL is 5 mg/mL, fifty times weaker. That is why a 50 mg dose of NAD+ is only 20 units while a 5 mg dose of something else can be 100. The draw is small because the solution is strong, not because the dose is.
It is also why the water volume is a genuine trade-off here rather than a convenience. More water lowers the concentration, which is what most people report helps with the sting, but it makes the draw bigger: 100 mg from a 500 mg vial is 40 units at 2 mL and 100 units at 5 mL. Past that you run out of syringe.
Common questions
- How many units is 100 mg of NAD+?
- 40 units on a U-100 syringe from a 500 mg vial reconstituted with 2 mL of bacteriostatic water, which is 250 mg/mL. From a 1,000 mg vial with 5 mL it is 50 units, and from a 500 mg vial with 5 mL it is 100 units, a full syringe.
- What is a common NAD+ dose per day?
- Reports cluster at 50 to 100 mg a day, which is 20 to 40 units from a 500 mg vial in 2 mL, with people often starting nearer 25 mg. There is no licensed dose; it is not an approved injectable medicine.
- How much NAD+ per week?
- At 50 mg daily, 350 mg a week, so a 500 mg vial lasts ten days. At 100 mg daily it is 700 mg a week and a 500 mg vial is five days. Three times a week at 100 mg is 300 mg a week.
- How often is NAD+ injected?
- Once daily and three times a week are the two patterns that get reported, usually in blocks of one to two weeks rather than continuously. Splitting a weekly total into more, smaller, more dilute injections is the change people describe making when the injection-site sting is the problem.
- How much bacteriostatic water for a 500 mg NAD+ vial?
- 2 mL is the usual choice and gives 250 mg/mL, so 50 mg is 20 units and 100 mg is 40 units. More water lowers the concentration, which is what most people report helps with the sting, at the cost of a bigger draw: at 5 mL, 100 mg is a full 100 units.
Common conversions
The same three-step maths, pre-filled for the doses people ask about most. Units on a U-100 insulin syringe, where 100 units is 1 mL.
- mg to units calculator Any dose at any concentration, both directions, with the full reference tables
- Units to mg calculator What the units you drew are in milligrams
- 1 mg to units The most-asked conversion, at every common setup
- 2 mg to units Retatrutide starting dose
- 2.5 mg to units Tirzepatide starting dose
- 5 mg to units Tirzepatide step two, MOTS-c, epitalon
- 10 mg to units Tirzepatide step four
- 12 mg to units Retatrutide top dose, tirzepatide 12.5 mg
- 15 mg to units Tirzepatide top dose
All calculators and charts
Weight management (GLP-1 family)
- Retatrutide calculator Vial, water and weekly dose to syringe units
- Tirzepatide calculator The 2.5 to 15 mg ladder, converted
- Semaglutide calculator The 0.25 to 2.4 mg ladder, converted
- Cagrilintide calculator Amylin analogue, 0.3 to 4.5 mg weekly
Growth hormone axis
- HGH (somatropin) calculator IU vials to syringe units
- Ipamorelin calculator mcg doses, in mL and units
- CJC-1295 (no DAC) calculator No-DAC and DAC, mcg to units
- CJC-1295 / Ipamorelin blend calculator The 5 mg + 5 mg blend, per component
- Sermorelin calculator Nightly mcg doses to units
- Tesamorelin calculator 1 to 2 mg daily, in units
- Hexarelin calculator mcg doses to units
- IGF-1 LR3 calculator Tens of mcg from very small vials
- AOD-9604 calculator 300 mcg daily, in units
- MOTS-c calculator 5 to 10 mg, two or three times a week
Repair, skin and blends
- BPC-157 calculator mcg doses from a 5 or 10 mg vial
- TB-500 calculator Loading and maintenance, in units
- BPC-157 / TB-500 blend calculator The Wolverine stack, per component
- GHK-Cu calculator 50 to 200 mg vials, mg doses
- GLOW blend calculator The 70 mg blend, split per component
- KLOW blend calculator The 80 mg blend, split per component
Other compounds
- PT-141 calculator 0.5 to 2 mg, as needed
- Melanotan II calculator mcg loading doses to units
- NAD+ calculator 100 to 1,000 mg vials, 25 to 200 mg doses
- Thymosin alpha-1 calculator 1.6 mg twice weekly, in units
- Epitalon calculator Short courses of 5 to 10 mg
- Kisspeptin-10 calculator mcg doses to units
- Glutathione calculator Hundreds of mg, or a 200 mg/mL solution
Dosage charts and schedules
- Peptide reconstitution chart Concentration for every vial and water combination
- Peptide dosing chart Printable unit tables for every compound
- Retatrutide titration schedule 2 to 12 mg in units, stage by stage
- Tirzepatide titration schedule 2.5 to 15 mg in units, stage by stage
- Semaglutide titration schedule 0.25 to 2.4 mg in units, stage by stage
- KLOW dosage and frequency chart The 80 mg blend at 2, 3 and 4 mL, both phases
- GLOW dosage chart The 70 mg blend, units to each peptide
- Wolverine stack dosing chart BPC-157 + TB-500, blend and separate vials
- MOTS-c dosage chart 5 and 10 mg vials at three water volumes
- Tirzepatide reconstitution chart Every vial size, every water volume, the whole ladder
- Retatrutide dosing chart 1 to 12 mg across 10, 20 and 30 mg vials
- Tirzepatide units to mg chart Both directions, every vial and water volume
- Semaglutide units to mg chart The dosage chart in units, both directions
- NAD+ injection dosage chart (this page)
- Tesamorelin dosage chart 2, 5 and 10 mg vials, 0.5 to 2 mg in units
- BPC-157 dosage chart mcg doses from 5 and 10 mg vials, four water volumes
- PT-141 dosage chart The 10 mg vial, 0.5 to 2 mg, with uses per vial
- Sermorelin dosage chart Nightly mcg doses from 5, 10 and 15 mg vials
- Printable dosing charts Four one-page charts to save, pin or print
- Peptide storage chart How long powder and mixed vials last, fridge to freezer
Tools
- Peptide calculator The general tool, any compound
- Reconstitution calculator How much water to add, and why it matters
- Bacteriostatic water calculator Pick a volume that lands where you can read it
- How to read an insulin syringe U-100 vs U-40, and the 2.5x error
- The Protocol Pack Free 25-page printable reference
- mg to units calculator Both directions, any concentration, with the reference tables
- 1 mg to units on an insulin syringe The conversion table for every common concentration
- Units to mg calculator The mark you drew, back into milligrams
- 2 mg to units on an insulin syringe 2 mg at every common vial and water volume
- 2.5 mg to units on an insulin syringe 2.5 mg at every common vial and water volume
- 5 mg to units on an insulin syringe 5 mg at every common vial and water volume
- 10 mg to units on an insulin syringe 10 mg at every common vial and water volume
- 12 mg to units on an insulin syringe 12 mg at every common vial and water volume
- 15 mg to units on an insulin syringe 15 mg at every common vial and water volume
- Methodology Every formula, assumption and rounding rule
- About Peptulator Who publishes it, independence and contact
- Embed the calculator One line of HTML, free, credit line only