PeptulatorReconstitution and dosing maths

NAD+ dosage calculator

NAD+ is sold in 100 to 1,000 mg vials and dosed in tens of milligrams, so the numbers look nothing like a peptide.

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Dose to units at the usual vial and water

DoseU-100 unitsDrawDoses per vialVial lasts
25 mg10 units0.1 mL2020 days
50 mg20 units0.2 mL1010 days
75 mg30 units0.3 mL66 days
100 mg40 units0.4 mL55 days
150 mg60 units0.6 mL33 days
200 mg80 units0.8 mL22 days

500 mg vial in 2 mL of bacteriostatic water (250 mg/mL), U-100 insulin syringe, once daily.

About NAD+

NAD+ is a coenzyme, not a peptide, but it is sold by the same vendors as a lyophilised powder for subcutaneous injection and the arithmetic is identical. It is not an approved injectable medicine. Flushing, nausea and injection-site pain are widely reported, and the pain is dose- and concentration-dependent.

A 500 mg vial with 2 mL of water is 250 mg/mL, so 50 mg is 0.2 mL, or 20 units. Because the site pain tracks concentration, some people reconstitute with more water and accept a larger draw. Commonly reported doses are 50 to 100 mg, daily or a few times a week, often starting lower.

The same 500 mg vial at other water volumes

If the draw is awkward to read, change the water, not the dose. The dose stays the same; only the mark moves.

VialBAC waterConcentrationDrawU-100 unitsDoses/vial
500 mg1 mL500 mg/mL0.1 mL10 units10
500 mg2 mL250 mg/mL0.2 mL20 units10
500 mg3 mL166.67 mg/mL0.3 mL30 units10
1000 mg1 mL1000 mg/mL0.05 mL5 units20
1000 mg2 mL500 mg/mL0.1 mL10 units20
1000 mg3 mL333.33 mg/mL0.15 mL15 units20

All rows above assume a 50 mg dose and a standard U-100 insulin syringe, where 100 units = 1 mL.

How long it lasts in the body

Cleared within minutes to hours; the injected molecule itself is not what persists. A decay curve is only drawn where a human half-life is documented, so there is none here. Treat any precise figure for NAD+ elsewhere with caution.

Common questions

How many units is 50 mg of NAD+?
It depends entirely on how much water you added. In a 500 mg vial reconstituted with 2 mL, the concentration is 250 mg/mL, so 50 mg is 0.2 mL, which is 20 units on a U-100 insulin syringe. In the same vial with 1 mL of water it is 10 units, and with 3 mL it is 30 units.
How much bacteriostatic water for a 500 mg NAD+ vial?
2 mL is the usual choice. It gives 250 mg/mL, which puts the commonly reported range of 25 mg to 200 mg between roughly 10 and 80 units. Aim for a draw between about 10 and 50 units, where the barrel is easiest to read, and add or remove water to get there.
How many doses are in a 500 mg vial?
Divide 500 by your dose. At 50 mg that is 10 doses, which at once daily is 10 days.
How many units is 100 mg of NAD+?
In a 500 mg vial with 2 mL of water, 40 units on a U-100 syringe. In a 1,000 mg vial with 5 mL, 50 units.
IndependentThis site does not sell peptides and is not owned by a vendor.
The maths is openThe formula is on every page and in the methodology. The code is one file with an automated test suite, run before each deploy.
How it earnsAffiliate links on one marked page, never inside the calculator.
ReviewedDose ranges and half-lives checked 3 September 2026.

Buying NAD+?

The where to buy page lists the vendors this site uses and what to check first. Peptide Bureau keeps the ranked scorecard.

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Tools

Before you use these numbers This is a unit-conversion tool. It turns a vial strength and a water volume into a concentration, a draw volume and a syringe mark. It does not tell you what to take, whether to take it, or whether a compound is safe for you. Most compounds listed are sold for laboratory research and are not approved for human use. Nothing here is medical advice. Dosing decisions belong with a licensed clinician who knows your history. Always check the strength printed on your own vial rather than trusting a preset.